The word ‘domestication’ conjures images of dogs, cats, cows, and other pets and farmyard animals, descendants of wild ancestors from thousands of years ago. Surprisingly, the humble yeast that ferments your beer and leavens your bread is also a domesticated organism: a little critter by the name of Saccharomyces cerevisiae, also known as Brewer’s or…
Plasmid vectors are ubiquitous in modern biological research and are produced in quantity by culture and subsequent extraction and purification from the bacterial host E. coli. This post outlines how to properly culture plasmid-hosing E. coli to ensure consistently high yields of plasmid vectors. Plasmid vectors are an essential, ubiquitous, and powerful tool in modern…
Plasmid vectors are key to modern biological research but can be prone to misidentification and loss through a variety of laboratory mishaps. This post outlines some easy steps you can take to confidently recover lost or misidentified plasmid vectors. Plasmid vectors are an essential, ubiquitous, and powerful tool in modern life science research, yet they…
