The end of a plasmid vector’s journey is purification and quantification. Reliable purification of high-quality plasmid vector DNA is straightforward using commercially available kits and a few extra tips. This, and an understanding of how to use UV-visual spectroscopy of DNA solutions to assess quantity and purity, will equip you with all the key skills to ensure quality plasmid vector DNA preparations.
Plasmid vectors are ubiquitous in modern biological research and are produced in quantity by culture and subsequent extraction and purification from the bacterial host E. coli. This post outlines how to properly culture plasmid-hosing E. coli to ensure consistently high yields of plasmid vectors. Plasmid vectors are an essential, ubiquitous, and powerful tool in modern…
Plasmid vectors are key to modern biological research but can be prone to misidentification and loss through a variety of laboratory mishaps. This post outlines some easy steps you can take to confidently recover lost or misidentified plasmid vectors. Plasmid vectors are an essential, ubiquitous, and powerful tool in modern life science research, yet they…
